2. Signaling Pathways
  3. Cell Cycle/DNA Damage
  4. DNA/RNA Synthesis

DNA/RNA Synthesis

DNA/RNA Synthesis

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RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-104014
    DMT-2'O-MOE-rG(ib) Phosphoramidite
    DMT-2'O-MOE-rG(ib) Phosphoramidite belongs to cyanoethyl-protected nucleoside phosphoramidites. DMT-2'O-MOE-rG(ib) Phosphoramidite is a derivative of nucleotide and guanosine. DMT-2'O-MOE-rG(ib) Phosphoramidite can be used for the stereochemical synthesis of phosphorothioate oligonucleotides.
    DMT-2'O-MOE-rG(ib) Phosphoramidite
  • HY-B0843A
    Metalaxyl-M
    		Inhibitor 98.01%
    Metalaxyl-M ((R)-Metalaxyl) is an orally active and selective inhibitor of fungal RNA polymerase, which exerts fungicidal activity by selectively interfering with the synthesis of fungal ribosomal RNA. Metalaxyl-M can also be used to induce inflammation in hepatocytes and regulate tryptophan metabolism. Metalaxyl-M can be used in ecotoxicology studies.
    Metalaxyl-M
  • HY-B1941
    4-tert-Octylphenol
    		Inhibitor 99.70%
    4-tert-Octylphenol, a endocrine-disrupting chemical, is an estrogenic agent. 4-tert-Octylphenol is also a biodegradation product of non-ionic surfactants alkylphenol polyethoxylates. 4-tert-Octylphenol induces apoptosis in neuronal progenitor cells in offspring mouse brain. 4-tert-Octylphenol reduces bromodeoxyuridine (BrdU), mitotic marker Ki67, and phospho-histone H3 (p-Histone-H3), resulting in a reduction of neuronal progenitor proliferation. 4-tert-Octylphenol disrupts brain development and behavior in mice, which is promising for reserch of immune response, neuro-related diseases and ethology.
    4-tert-Octylphenol
  • HY-W265757
    4-Ethylcatechol
    		Inhibitor 99.50%
    4-Ethylcatechol is a ring-dihydroxylated metabolite of 4-Ethylphenol that leads to oxidative DNA damage.
    4-Ethylcatechol
  • HY-44358
    Remdesivir nucleoside monophosphate
    		Inhibitor 99.98%
    Remdesivir nucleoside monophosphate is a metabolite of Remdesivir. Remdesivir is a nucleoside analogue with effective antiviral activity against SARS-CoV and MERS-CoV.
    Remdesivir nucleoside monophosphate
  • HY-164496
    KL-50
    		Inhibitor 99.82%
    KL-50 is a selective toxin toward tumors that lack the DNA repair protein O6-methylguanine-DNA-methyltransferase (MGMT), which reverses the formation of O6-alkylguanine lesions. KL-50 activates DNA damage response pathways and cycle arrest in MGMT-cells, independent of mismatch repair (MMR). KL-50 is promising for research of brain tumors that lack the DNA repair protein MGMT.
    KL-50
  • HY-N1150S8
    Thymidine-13C10,15N2
    		99.90%
    Thymidine-13C10,15N2 is the 13C and 15N labeled Thymidine[1]. Thymidine, a specific precursor of deoxyribonucleic acid, is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication[2][3][4].
    Thymidine-<sup>13</sup>C<sub>10</sub>,<sup>15</sup>N<sub>2</sub>
  • HY-W173146
    ALKBH5-IN-4
    ALKBH5-IN-4 (compound 3) is a RNA m6A demethylase ALKBH5 inhibitor with an IC50 of 0.84 μM. ALKBH5-IN-4 shows inhibitory effect on leukemia cells[1].
    ALKBH5-IN-4
  • HY-108999A
    Crisnatol
    		Inhibitor 99.51%
    Crisnatol (BWA770U) is an orally active and anticancer agent, and a member of the arylmethylaminopropanediol class of DNA intercalators. Crisnatol shows in vitro cytotoxicity against human breast cancer cells, but not normal human skin fibroblasts.
    Crisnatol
  • HY-B0152R
    Adenine (Standard)
    		Chemical
    Adenine (Standard) is the analytical standard of Adenine. This product is intended for research and analytical applications. Adenine (6-Aminopurine), a purine, is one of the four nucleobases in the nucleic acid of DNA. Adenine acts as a chemical component of DNA and RNA. Adenine also plays an important role in biochemistry involved in cellular respiration, the form of both ATP and the cofactors (NAD and FAD), and protein synthesis.
    Adenine (Standard)
  • HY-138603
    5'-O-DMT-N4-Bz-2'-F-dC
    		Activator 99.85%
    5'-O-DMT-N4-Bz-2'-F-dC is a nucleoside with protective and modification effects.
    5'-O-DMT-N4-Bz-2'-F-dC
  • HY-W570885
    2'-O-MOE-rC
    		≥98.0%
    2'-O-MOE-rC is a 2'-O-MOE modified nucleoside. 2'-O-MOE-rC can be used for synthesis of DNA.
    2'-O-MOE-rC
  • HY-16398
    Pipobroman
    		Inhibitor 99.81%
    Pipobroman is a bromide derivative of piperazine and acts as an alkylating agent. Pipobroman plays its role by inhibiting DNA and RNA polymerase or by reducing pyrimidine nucleotide incorporation into DNA. Pipobroman can be used for the cancer research, including polycythemia vera, myeloproliferative neoplasm, and AML et.al.
    Pipobroman
  • HY-145330
    NSC639828
    		Inhibitor 99.91%
    NSC639828 is a potent inhibitor of DNA polymerase α with an IC50 of 70 μM. NSC639828 has high antitumor activity. NSC639828 has the potential for researching cancer disease.
    NSC639828
  • HY-W019815
    N-Ethyl-N-nitrosourea
    		Inhibitor 99.92%
    N-Ethyl-N-nitrosourea (ENU) is a DNA alkylating agent. N-Ethyl-N-nitrosourea induces leukemia by alkylating nucleobases, disrupting DNA, and resulting in bone marrow suppression and the formation of leukemic cells. N-Ethyl-N-nitrosourea is teratogenic in vivo, inducing tumor formation and paw malformations in pregnant rats. N-Ethyl-N-nitrosourea cause central nervous system (CNS) tumors and genetic disorders.
    N-Ethyl-N-nitrosourea
  • HY-E70529
    Ribonucleoside vanadyl complexes
    Ribonucleoside vanadyl complexes is a ribonuclease (RNase) inhibitor that can be used in RNA isolation and detection to inhibit RNA degradation.
    Ribonucleoside vanadyl complexes
  • HY-124404A
    12(S)-HETE
    		Inducer 99.10%
    12(S)-HETE is the 12-lipoxygenase metabolite of arachidonic acid and has a mitogenic effect on cancer cell proliferation. 12(S)-HETE induces tyrosine phosphorylation of cellular proteins, promotes ERK and P38 MAPK phosphorylation, increases DNA synthesis, and stimulates the proliferation of pancreatic cancer cells.
    12(S)-HETE
  • HY-165099
    C-8 Ceramide-1-phosphate
    		Inducer ≥99.0%
    C-8 Ceramide-1-phosphate is a cell apoptosis inhibitor and a cell survival inducer that can stimulate DNA synthesis and cell division. C-8 Ceramide-1-phosphate can inhibit acidic sphingomyelinase (SMase) and stimulate PI3-K, which in turn produces PIP3; PIP3 can also inhibit acidic SMase. The C-8 Ceramide-1-phosphate and ceramide can be interconverted in cells through kinase and phosphatase activity, and maintaining the balance between the two is crucial for cellular and tissue homeostasis.
    C-8 Ceramide-1-phosphate
  • HY-131605B
    Ganciclovir triphosphate disodium
    		Inhibitor 98.65%
    Ganciclovir triphosphate (GCV-TP) disodium is a synthetic 2'-deoxyguanosine analog with activity in inhibiting human cytomegalovirus (CMV) replication. Ganciclovir triphosphate disodium is effective against CMV infection by binding to viral DNA polymerase and interfering with viral DNA synthesis. Ganciclovir triphosphate disodium has an IC50 of 0.01 μM against CMV strains from humans, monkeys, mice, and guinea pigs. Ganciclovir triphosphate disodium has also been used in gene-directed enzyme prodrug inhibition for cancer inhibition.
    Ganciclovir triphosphate disodium
  • HY-163941
    GSK_WRN2
    GSK_WRN2 is a potent and selective covalent WRN helicase inhibitor that suppresses replication stress caused by DNA (TA)n dinucleotide repeat expansions. GSK_WRN2 can be used to study microsatellite instability (MSI) cancers.
    GSK_WRN2
Cat. No. Product Name / Synonyms Application Reactivity